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测定甲胎蛋白的非标记电位型免疫传感器

Potentiometric Determination of α-Fetoprotein (AFP) Using a Non-labeled Antibody Immunosensor

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【作者】 彭图治祝方猛杨丽菊何巧红程琼

【Author】 Peng Tuzhi; Zhu Fangmeng; Yang Liju; He Qiaohong; Cheng Qiong(Department of Chemistry,Hangzhou University,Hangzhou 310028)

【机构】 杭州大学化学系!杭州310028

【Abstract】 The anti-AFP antibody is immobilized on the surface of PVC-BSA(bovine serumalbumin) membrane, which has been activated by NH2OH·HCl solution. The immunosen-sor consists of such a membrane with added AFP and Ag-AgCl internal electrode. It showedlinear response over concentration range of 10-300μg/L and potential difference(△E) of 15~20 mV for 300μg/L of AFP. Other common antigens in human serum do not interfere withAFP’s determination.

【关键词】 甲胎蛋白电位法免疫传感器
【Key words】 α-fetoproteinpotentiometryimmunosensor
【基金】 浙江省自然科学基金;分析测试基金
  • 【文献出处】 应用化学 ,CHINESE JOURNAL OF APPLIED CHEMISTRY , 编辑部邮箱 ,1998年01期
  • 【分类号】O646
  • 【被引频次】48
  • 【下载频次】137
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