【摘要】 从富集培养物中筛选到以甲胺磷为唯一碳源生长的菌株ＮＭＪ５和以乐果为唯一碳源生长的菌株ＮＭＬ３，经鉴定分别为芽孢杆菌属（Ｂａｃｉｌｕｓｓｐ．）和假单胞菌属（Ｐｓｅｕｄｏｍｏｎａｓｓｐ．）．它们利用甲胺磷、乐果生长的最适条件及在无机盐培养基中农药最大耐受浓度分别为ｐＨ７．５，ｔ＝３５℃，ρ＝１０００ｍｇ／Ｌ和ｐＨ７．５，ｔ＝３０℃，ρ＝２０００ｍｇ／Ｌ．在无碳基础培养基内单菌株培养８ｄ，５２４ｍｇ／Ｌ的甲胺磷好气降解４２．５％，厌气降解３５．９％，２５０ｍｇ／Ｌ的乐果好气降解５０．２％，厌气降解１６．４％．小区试验表明，ＮＭＪ５、ＮＭＬ３的菌液制剂对普通白菜的变种南农矮脚黄（Ｂｒａｓｉｃａｃａｍｐｅｓｔｒｉｓｓｐ．ｃｈｉｎｅｎｓｉｓＬ．ｖａｒ．ｃｏｍｍｕｎｉｓＴｓｅｎｅｔＬｅｅ）中残留的甲胺磷、乐果有明显的去除作用．更多还原

【Abstract】 A methamidophos (MMP) degrading strain NM J 5 and dimethoate(DMA) degrading strain NM L 3 were isolated and selected from soils and sludges. NM J 5 and NM L 3 were identified as Bacillus sp. and Pseudomonas sp. respectively. The optimum temperature, pH and maximum tolerance concentration to grow on MMP by NM J 5 were 35℃,7.5 and 1 000 mg/L,and those to grow on DMA by NM L 3 were 30℃,7.5 and 2 000 mg/L respectively. The aerobic and anaerobic degradation rates of MMP at the concentration of 524 mg/L by NM J 5 within 8 days were 42.5% and 35.9%. Similarily,those of DMA at the concentration of 250 mg/L by NM L 3 were 50.2% and 16.4% respectively. The results from the field experiments in small areas showed that NM J 5 and NM L 3 had obvious effects on the removal of residual MMP and DMA in Chinese cabbage (Brassica campestris ssp. chinensis L.var. communis Tsen et Lee).更多还原