【摘要】 对膜荚黄芪毛状根中６种异黄酮成分进行了反相高效液相色谱法测定。色谱柱为ＮｕｃｌｅｏｓｉｌＣ１８柱，流动相为甲醇—水（３∶２和３∶１，ｖ／ｖ），检测波长为２５４ｎｍ和２８０ｎｍ。６种异黄酮成分为：１０羟基３，９二甲氧基紫檀烷，（３Ｒ）８，２’二羟基７，４’二甲氧基异黄烷，芒柄花素（７羟基４’甲氧基异黄酮），８，３’二羟基７，４’二甲氧基异黄酮，２’羟基３’，４’二甲氧基异黄烷７Ｏ葡萄吡喃糖甙，毛蕊异黄酮（７，３’二羟基４’甲氧基异黄酮）。异黄酮浓度在２５～１２５μｇ范围内与峰面积有良好的线性关系；加样回收率为９６４７％～１０３３３％；精密度试验相对标准偏差为２５７％～６５２％；测得黄芪毛状根中６种异黄酮成分的含量在００００５％～０００６５％之间。更多还原

【Abstract】 An accurate RPHPLC method for the determination of 10hydroxy3,9dimethoxypeterocarpan, (3R)8,2’dihydroxy7,4’dimethoxyisoflavan, formononetin (7hydroxy4’methoxyisoflavone), 8,3’dihydroxy7,4’dimethoxyisoflavone,2’hydroxy3’,4’dimethoxyisoflavan7Oglucopyranoside and alycosin (7,3’dihydroxy4’methoxyisoflavone) in the hairy root cultures of Astragalus membranaceus was developed. Among them, 10hydroxy3,9dimethoxypeterocarpan, (3R)8,2’dihydroxy7,4’dimethoxyisoflavan and 8,3’dihydroxy7,4’dimethoxyisoflavone were new compounds in Astragalus membranaceus. The analytical column was Nucleosil C18. The mobile phases were methanol—water=3∶2 and 1∶1 (v/v). The detection wavelengths were 254 nm and 280 nm. Calibration graphs were rectilinear between 25 μg and 125 μg. The recoveries were between 9647% and 10333%. The relative standard deviations (RSDs) of mesurement precision test were 257%～652%. The flavonoid contents in the hairy root cultures were between 00005% and 00065% (dry weight).更多还原