【摘要】 以怀孕为３，５，７ｄ的昆明种小鼠的卵巢为材料，利用质粒扩增的、地高辛标记的基因探针在组织切 片上进行ＤＮＡ－ｍＲＮＡ分子原位杂交，研究了 ｃｙｃｌｉｎＤ１，ＰＣＮＡ． ｃｄｃ２，ｐ２１和 ｐ１６基因共 ５种细胞周期调控基因 在怀孕早期小鼠卵巢中的表达．结果发现：ｐ２１基因在怀孕的 ３， ５和 ７ ｄ的表达都较弱； ＰＣＮＡ基因和 ｐ１６基因 的表达强度随孕期的延长而减弱； ｐ２１和 ｃｄｃ２基因和ｃｙｃｌｉｎ Ｄ１基因的表达强度以 ３， ７ ｄ的为强，而怀孕 ５ ｄ的 极弱，其表达强度呈现了高（３ｄ）→低（５ｄ）→高（７ｄ）的波浪型变化；ＰＣＮＡ基因的表达变化与其在离体细胞中 的 ＤＮＡ合成功能一致，而ｃｙｃｌｉｎ Ｄ１， ｃｄｃ２，ｐ２１和 ｐ１６基因表达变化与其细胞周期变化无关，因此它们在孕鼠卵 巢中的功能可能不是作为细胞周期调控因子，而可能与某些基因的表达激活或关闭有关．这些事实一方面表明 细胞周期调控因子在卵巢中的作用可能与细胞周期调控无关，另一方面也说明卵巢组织在怀孕初期在生理上 极为活跃，变化复杂．更多还原

【Abstract】 Using in situ hybridization technique with Dig Labeled DNA probe, we studied the expression of cyclin D1, PCNA, P21, p16, and cdc2 gene in the ovary in kunming mice being 3, 5, 7 days pregnant. The results shows that a) The expression strength of PCNA and p16 gene were descendent as pregnancy progress; b)The expression strength of P21,cdc2 and cyclin D1 gene were variational like waves, i. e. being strong in third and seventh day, while being weak in fifth day. The expression pattern of PCNA gene above mentioned do not constrast with the view that tis gene functions as a cell growth-promotive gene. Since the expression of cyclin D1,cdc2, P21 and p16 above described are inconsistent with role of them as the cell growth-promotive and the cell growth-inhibitory genes respectively, these genes may be related to the expression regulution of those genes except cell cycle-related genes. The waves-like variation of the expression of cdc2 and cyclin D1 genes suggests that the physiological change of mice ovarr during early stage pregnant is very complex and activation.更多还原