【摘要】 在粳稻花药培养过程中，花粉植株的诱导频率受接种材料的基因型、花粉发育时期和培养基成分等因素的影响．杂交种的诱导率高于纯合品种，接种最佳时期为花粉发育单核中晚期，诱导培养基以Ｎ６＋２．４—Ｄ２ｍｇ／ｌ＋ＫＴ０．５ｍｇ／ｌ＋蔗糖５％＋琼脂粉０．７％；分化培养基以Ｍｓ＋ＢＡ２ｍｇ／ｌ＋ＮＡＡ０．２—０．５ｍｇ／ｌ＋蔗糖３％＋琼脂粉０．７％；壮苗培养基以１／２ＭＳ＋微量＋铁盐＋ＮＡＡ０．１—０．２ｍｇ／ｌ＋多效唑２ｍｇ／ｌ＋蔗糖２％＋琼脂０．７％为最好更多还原

【Abstract】 In the anther culture of Japonica Rice,the induction rate of pollen plantlets was affected by the genotypes of materials, developmental stage of anthers,formulae of culture medium and so on. Hybrids had a higher induction rate as compared with homozygous varieties.The suitable developmental stage of the microspores in the anthers was in the mid-or-late-uninucleate.Better medium formulae were N6+2mg/l2.4-D+0.5mg/l KT+5% sucrose+0.7% agar for dedifferentiation and Ms+2mg/lBA+0.2-0.5mg/l NAA+3% sucrose+0.7%agar for differentiation.To make the plantlets strong,you should transfer them onto 1/2 Ms (allFe-salt)+0.1-0.2mg/l NAA+2mg/l MET+2% sucrose+0.7% agar.更多还原