【摘要】 建立了用柱切换测定血浆中兰索拉唑的方法。血样用蒸馏水简单稀释后注入填充ＬｉＣｈｒｏｍｐｒｅｐＲＰ２（２５～４０μｍ）的预柱上。用蒸馏水冲洗出血浆中蛋白质和其它极性成分。切换后，浓缩在预柱上的兰索拉唑被流动相甲醇０２ｍｏｌ·Ｌ１醋酸铵（６５：３５）反冲到分析柱ＳｈｉｍｐａｃｋＣＬＣＯＤＳ上进行分析。预柱用净化液进行消除和再生。本法有很好的精密度和回收率，检测限为０００５ｍｇ·Ｌ１，日内和日间测定相对标准差小于２５％和５３％。此分析方法已成功地用于测定自愿受试者的兰索拉唑药代动力学更多还原

【Abstract】 High performance liquid chromatography with column switching has been developed for the determination of lansoprazole in plasma. The plasma samples were injected onto a pretreatment column packed with LiChromprep RP2 (25～40 mm) after simple dilution with distilled water. Distilled water was used to wash out protein and other polar components in plasma. After switching, the concentrated lansoprazole was eluted in the backflush mode onto a Shimpack CLC ODS column with methanol 0 2 mol·L 1 ammonium acetate (65:35) as mobile phase. Purge solutions were used for clean up and for regenerating the pretreatment column. The method showed good precision and recovery. The detection limit was 0 005 mg·L -1 plasma. The RSD’s (intra and interday) were less than 2 5% and 5 3% respectively. The method has been successfully used to determine pharmacokinetics of lansoprazole in Chinese volunteers.更多还原