【摘要】 为了探讨新生儿缺氧缺血性脑病（HIE）脑损伤时，是否存在细胞死亡的另一种形式—凋亡（apoptosisApo），选用48只健康新生7天的SD大鼠，建立HIE标准化动物模型，将其随机分为对照组和缺氧缺血后1、4、18、24、40、72h、7天组。应用HE染色、电镜、组织切片原位末端标记技术（TUNEL）等手段，观察和比较各组实验侧脑皮质细胞的凋亡形态、特点和密度。结果光镜和电镜下可见细胞皱缩、核碎裂、Apo小体；原位标记显示实验侧皮质、海马回、丘脑均有末端标记阳性的细胞，证实HIE中的Apo现象。8组皮质区阳性细胞数／mm2F＝109．21P＜0．001。两两比较结果：各级之间P＜0．001。说明缺氧缺血可引起脑细胞凋亡，其强度与细胞凋亡有明显统计学意义。其中在皮质区18h点标记细胞最明显，Apo出现早于坏死，在缺氧缺血脑损伤的早期。提示机体在遭遇病理因素时，首先启动自身内部机制，让部分细胞主动性的死亡。更多还原

【Abstract】 To investigating another form of brain cell death-Apoptosis during cerebral cell injury in the traditioal model of Hypoxia-ischemia encephalopathy (HIE), we observed the histological features, time course and density of apoptotic cell and compared in the ipsilateral hemisphere after permanent ischemia 1, 4, 18, 24, 40, 72h, 7tays following 3h hypoxia with control-group by Using terminal deoxynuleotidyl transferase-mediated dUTP nick end labelling (TUNEL), HE staining and Electron-microscope technique. Result showed that positive cells identified by in situ labelling in the cortex of the ipsilateral hemisphere indicate apoptosis in HIE. By quantitative analysis showing there are atatistical significant defference among 8 groupes appotosis cell/mm2. (P<0. 001) and peak for apoptosic cell in the cortex is 18h point更多还原