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用乙型肝炎表面抗原地衣红染色法检测兔出血症病毒

DETECTION OF RABBIT HEMORRHAGIC DISEASE VIRUS BY HEPATITIS B SURFACE ANTIGEN ORCEIN STAINING

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【作者】 佘锐萍杨汉春马毅新高齐瑜

【Author】 She Ruiping, Yang Hanchun, Ma Yixin, Gao Qiyu(Veterinary College,China AgriculturalUniversity,Beijing 100094)

【机构】 中国农业大学动物医学院

【摘要】 本研究采用乙型肝炎表面抗原地衣红染色法对30例实验感染兔出血症病毒(RHDV)的兔及10例健康对照兔的肝脏进行切片染色观察,并与免疫胶体金抗体标记及SAB免疫组织化学染色结果相比较。结果表明,在感染兔的肝细胞核及细胞浆内可见到颗粒状暗棕色的地衣红染色阳性物质,阳性物质的分布及着色强度与免疫组织化学染色结果相一致。健康对照兔的肝细胞内未见到地衣红阳性反应物质。结果提示:RHDV可能与人类乙型肝炎病毒在抗原性上有一定的相关性。另外,此方法省时、省经费、简便易行,可用于兔出血症的快速诊断

【Abstract】 ? In this study,hepatitis B surface antigen orcein staining was applied for observing the livers of 10 control rabbits and 30 rabbits infected with rabbit hemorrhagic disease virus And it’s result was compared with that of immunogold staining.The result showed that orcein positive reaction material,which was particle or mass in shape and dard brown in colour,was found in the nuclei and cytoplasma of liver cellsThe distribution of the material and its staining intensity were identical with those in immunogold stainingThe liver of control rabbit showed negative reactionThe result suggests that there should be some common ground on the antigen component between rabbit hemorrhagic disease virus and human hepatitis B virusThis method was simple,specific,timesaving and low cost,so it can be practised in common laboratoriesThe process can be finished within 2 hours if frozen section was used

  • 【文献出处】 畜牧兽医学报 ,Chinese Journal of Animal and Veterinary Sciences , 编辑部邮箱 ,1998年01期
  • 【分类号】S858.291
  • 【被引频次】12
  • 【下载频次】46
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