节点文献
链霉菌种间融合及融合产物的分析
INTERSPECIFIC PROTOPLAST FUSION OF STREPTOMYCES ANDTHE IDENTIFICATION OF FUSANTS
【摘要】 采用灭活龟裂链霉菌原生质体与金霉素链霉菌原生质体在聚乙二醇诱导下进行原生质体种间融合,利用金霉素链霉菌对链霉素的敏感性,建立选择培养基,提高了融合子的检出率。并采用同工酶谱分析及抗生素效价测定,对融合子进行了初步鉴定
【Abstract】 This paper presented the interspecific fusion of alive protoplasts of streptomyces aureofaciens and inactivated protoplasts of streptomyces rimous.Based on the sensitivity of streptomyces aureofaciens to streptomycin the selectiue medium was formed,which raised the efficiency of picking out fusants.By analysing the antibioticproducing obility and isozyme patterns,fusants were indentified.
【关键词】 金霉素链霉菌;
龟裂链霉菌;
原生质体融合;
【Key words】 streptomyces aureofaciens streptomyces rimosus protoplast fusion;
【Key words】 streptomyces aureofaciens streptomyces rimosus protoplast fusion;
- 【文献出处】 西安矿业学院学报 ,Journal of Xi’an Mining Institute , 编辑部邮箱 ,1998年02期
- 【分类号】Q949.32,Q936
- 【被引频次】3
- 【下载频次】82