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用斑点酶免疫试验检测类风湿因子

DETECTING RHEUMATOID FACTOR BY DOT-ENZYME IMMUNOASSAY

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【作者】 俞红吉; 潘培根; 杨继虞; 严可宁; 曾蓉; 师丽丽; 陈云科; 门菊莲; 田兰英;

【Author】 Yu Hongji(Department of Clinical Epidemiology of West China university of Medical Sciences, Chengdu 610041)

【机构】 华西医科大学附一院临床流行病教研室!成都; 610041; 华西医科大学附一院医学检验教研室; 华西医科大学药学院生化教研室!成都; 610941; 陕西省渭南市卫生防疫站检验科!渭南市; 714000; 山西省运城地?;

【摘要】 应用斑点酶兔疫试验和免疫胶乳凝集试验对60份门诊待测类风湿因子的病人血清进行了3次对比检测,实验结果表明,斑点酶免疫试验不仅重复性、稳定性、灵敏度较免疫胶乳试验好,而且能区分IgG型和IgM型类风湿因子,具有一定实用性。

【Abstract】 Sixty serum samples from outpatients were detected by boty dot-enzyme immunoassay (dot-EIA) and immune latex agglutination (ILA) for rheumatoid factor(RF). After the sixty serum samples were repeatedly examined three times, the results showed that not only the reproducibility, the stability and sensitivity of dot-EIA were better than those of ILA, but also the dot-EIA could differentiate between IgG-RF and IgM-RF. The dot-EIA was suitable for practical purposes.

【关键词】 类风湿因子; 斑点酶免疫试验; 免疫胶乳凝集试验;
【Key words】 RF; Dot-EIA; ILA;
  • 【文献出处】 现代预防医学 ,Modern Preventive Medicine , 编辑部邮箱 ,1998年03期
  • 【分类号】R446.6
  • 【被引频次】1
  • 【下载频次】30
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