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茄病菌诱导子对肉苁蓉愈伤组织氨基酸含量的影响

Effect of elicitor of Fasarium solani on the aminoacid contents of Cistanche deserticola callus

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【作者】 李森; 吴新; 董效成; 郑旭; 楼之岑;

【Author】 Li Sheng; Wu Xin; Dong Xiaocheng; Zheng Xu; Zheng Junhua and Lou Zhicen(School of Pharmaceutical Sciences, Beijing Medical University, Beijing 100083)

【机构】 北京医科大学药学院!北京; 100853;

【摘要】 从寄生药用植物肉苁蓉(CistanchedeserticolaMa)及寄主梭梭(HalaxylonammodendronBunge)根系中分离出真菌镰刀菌属茄病菌(Fasariumsolani)菌丝。在梭梭根系纵切片观察到木质部导管中有半透明菌丝。在MS培养基中,菌丝形成菌丝体,以菌丝体制成的诱导子(elicitor)能显著提高愈伤组织氨基酸含量,与野生植株相比,7种人体必需氨基酸(除苏氨酸略低外)均高于原植株,其中苯丙氨酸高达10倍以上。

【Abstract】 The white hyphae were isolated from the root systems of both the parasite plant Cistanchedeserticola and its host Halaxylon ammodendron Bunge, which were identified as Fasarium solani, aspectes of the genus Fasarium LK. The translucent hyphae were also observed in the vessel elements of xylem in the longitudinal section of Halaxylon ammodendron root system. When the hyphae cultured on the MS medium, the white mycelia were formed. The elicitor prepared from the mycelia could remarkedly improve the aminoacid contents in the Cistanche deserticola calli. The contents of seven human essential aminoacids except threonine were higher than that in the original plant, such as, the phenylananine content was tenfold higher.

【关键词】 诱导子; 肉苁蓉愈伤组织; 氨基酸;
【Key words】 elicitor; Cistanche deserticola callus; aminoacid;
【基金】 国家自然科学基金
  • 【文献出处】 西北药学杂志 ,NORTHWEST PHARMACEUTICAL JOURNAL , 编辑部邮箱 ,1998年06期
  • 【分类号】R284
  • 【被引频次】25
  • 【下载频次】125
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