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肉苁蓉的愈伤组织培养

Callus culture of Cistanche deserticola

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【作者】 吴新李森董效成郑旭郑俊华楼之岑

【Author】 Wu Xin; Li Sen; Dong Xiaocheng; Zheng Xu; Zheng Junhua and Lou Zhicen (School of pharmaceutical Science, Beijing Medical University, Beijing 100083)

【机构】 北京医科大学药学院!北京100083

【摘要】 报道由肉苁蓉(Cistanchedeserticola)不同部位诱导出愈伤组织,并筛选出适合于愈伤组织快速生长的最佳培养条件。结果表明:幼芽愈伤组织诱导率较种子高;愈伤组织生长的基本培养基以B5最好;适宜的激素种类和配比为IAA0.5mg/L和KT2mg/L。适宜的培养条件为pH6.0。

【Abstract】 The induction of callus from different parts of Cistanche deserticola and the optimum condi nons under which the callus grew fast were discussed in this paper. The results showed thai the induction rate of the buds was higher than that of the seeds. The B5 basic medium with the pH6.0. was the best one of all screened media. The callus grewth was promoted when 0.5 mg/L LAA and 2 mg/LKT were added in the media.

【关键词】 肉苁蓉愈伤组织诱导组织培养
【Key words】 Cistanche deserticolacallusinductiontissue culture
【基金】 国家自然科学基金
  • 【文献出处】 西北药学杂志 ,NORTHWEST PHARMACEUTICAL JOURNAL , 编辑部邮箱 ,1998年03期
  • 【分类号】R285
  • 【被引频次】39
  • 【下载频次】215
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