【摘要】 对测定糖化酶中葡萄糖苷转移酶（转苷酶）酶活的方法进行了研究。采用α－ＭＧ为底物，用ｋｎｉｇｈｔ－Ａｌｅｎ法测定转苷酶催化α－ＭＧ分解产生的葡萄糖，计算得转苷酶酶活值。通过条件试验，找到了底物浓度、碱性铜试剂用量、加热时间、滴定剂浓度等的最佳范围。并测定了３×１０４，５×１０４，６×１０４μｍｏｌ／ｍｉｎ糖化酶粉剂和发酵液中的转苷酶活性，做了酶法、光度法与该方法的对比试验。更多还原

【Abstract】 In this paper, a determination method of glucosyltransferase activity in saccharogenic enzyme is developed. Taking α MG as a substrate, glucose decomposed from α MG catalyzed by glucosyltransferas is determined with Knight Allen method and the activities of glucosyltransferase is calculated. By conditional test, the best limits are obtained for concerntration of the substrate, heating period, and concentration of titrant. The activities of glucosyltransferase in saccharogenic enzyme powder and fermentation liquid per 3000, 5000, 6000 unit are determined. The result suggests that this determination method has the advantages of accuracy, quickness and simplicity.更多还原