【摘要】 研究了６种表面活性剂单独或任意两种混合使用对火焰原子吸收光谱法测定血清铜的吸光度的影响。结果显示，聚乙烯醇单独或与其它表面活性剂混合使用均使吸光度减小，吐温－２０、乳化剂ＯＰ、溴化十六烷基三甲铵、阿拉伯树胶、十二烷基硫酸钠单独或混合使用均使铜的吸光度增加，其中吐温－２０与十二烷基硫酸钠混合使用效果最好，吸光度增加５０％，且空白值为零。建立了吐温－２０与十二烷基硫酸钠混合表面活性剂增敏火焰原子吸收光谱法测定血清微量铜的方法，相对标准偏差ＲＳＤ为１．３５％～２．１１％，回收率为９８．００％～９８．１３％，检出限为０．０５２４μｍｏｌ／Ｌ。更多还原

【Abstract】 The effects of six kinds of surfactants were studied in the detection of copper in serum by atomic absorption spectrometry.The results demonstrated that the absorption decreased when polyvinyl alcohol was used alone or together with the other one,and the absorption increased when one or two of Tween 20,emulgent OP,palmityl trimethyl ammonium bromide,arabicgum and sodium laurylsulfate were used.Tween 20 and sodium laurylsulfate used together are most effective.A method of determination of serum copper by atomic absorption spectrometry sensitized with mixture of Tween 20 and sodium laurylsulfate was established.The absorption increased by 50%.The relative standard deviation was 135%～211%.The recovery was 9800%～9813%.The detection limit was 00524μmol/L.更多还原