【摘要】 对不同来源的55株双歧杆菌进行了数值分类研究。这些菌包括13株参照菌株和42株新分离物。在这些菌株中19株来源于人体，25株来自动物源，11株分离自污水。有的菌株是从未曾报道过的动物来源分离到的。通过数值分析比较了75项性状（形态、生理生化和对抗生素敏感性等），以不加权平均链锁聚类的方式进行族群归类。在70％Sm水平上划分成5个聚类群和12个亚群。对这些聚类群菌株间的关系进行了分析。在数值分类树状图谱的簇群归类中，人和动物来源的菌株基本上彼此分开，而分离自污水的菌株穿插在人或动物来源的聚类群中。在数值分类的基础上对各聚类群中的一些菌株的DNA中G＋Cmol％进行了测定。依据16SrRNA可变区序列的分析，以PCR方法合成的生物素标记探针，对某些代表性菌株的DNA片段同源性进行了分析．同种的菌株间与不同种菌株间的同源性显示有差别。更多还原

【Abstract】 Fifty five sthans of bifldobacteria belonging to Bytdobacterium from various soarees were studied by using ntnnerical taxonoAnc techniques. These organisms included 13 reference sthens and 42 newly isolated sthans (19 sthens of hUInan sources, 25 sthans of edmal somees and 11 strains obtained frOm sewage). Several stalns isolated from the edmals weren’t isolated before. overall sindlallhes of the sthens for 75 characters contained morphological, Physiological and biocheAncal charactershcs and anhbacterial suscePtibility were detendned. Dendrognim of numerical analysis based on unweightod Pair cluster average linkage were achieved. Flve clusters were defined at 70% Sm level. The relahonships of these sthens among five clusters were analysed. The stalns of human sourees were basically separeted from the animal sthens, and the sewage strains were distributed betWeen the animal clusters and the human clusters. on the basis of the numerical analysis, the G+ C content of DNA of som representahve strains among these cltisters were measured. Biohn-labeled DNA segments, comPlementary to variable regions of 16S rRNA, were amplified using PCR as probes. In the dot-blot hybridizahon the relahonships of DNA segmentS among some strains were discussed. The test resultS showed that the homologies among the sthens in same species and various species were differenL The studies are valuable for furtrher researeh in the relahonships of some species axnong bifidobacteria of various sourees.更多还原