【摘要】 从土壤中分离到9株产β-甘露聚糖酶的芽孢杆菌（Bacillus sp. ）,Bacillus sp. M250ml三角瓶摇瓶培养试验,产酶最适条件为32～34℃、0.35％ Na₂CO₃、发酵60h 酶活力为100～140u·ml^-1。100L罐发酵,在30～32℃、1:0.75vvm通气量、200r·min^-1条件下,发酵液酶活力高达330u·ml^-1。酶的最适反应温度和pH分别为50℃和6.0。在低于50℃,pH5.0～7.0酶稳定。Fe³⁺、Al³⁺、EDTA、Hg²⁺对酶有抑制作用,Mn²⁺、Ba²⁺对酶有激活作用。更多还原

【Abstract】 Nine strains of Bacillus producing B-mannanase were isolated from soil. Some conditions for B-mannanase production from Bacillus sp. M50 was studied in shake flask (30ml in 250ml flask). The optimal cultivation condition for β-mannanase production were : Na2CO3 0. 35% ; temperature 32-34℃ and cultivation time for 60h and B-mannanase activity was 100-140u× ml.Fermentation of B-mannanase form M50 has been carried out in 100L tank. The temperature,ventilation quantity and agitation speed were 30-32℃ ; 1 : 0. 75vvm and 200r. min-1 respectively. The fermentation cycle was 28h and the hightest B-mannanase activity was 330u × ml-1. The optimal temperature and pH for β-mannanase reaction were 50 ℃ and 6. 0 respectively. It showed a hight stability at pH4. 0-7. 0 and temperature below 50℃. The activity of enzyme were strongly mhibned by Al3+.EDTA,Hg2+and slightly stimulated by Ba2+.Mn2+.更多还原