【摘要】 从明亮发光杆菌（A2）中分离得到一种自发暗变株（S1），其表型发光强度小于A2的1／10000。溴化乙锭（EB）（0．6μg／ml）在24h内能强烈地诱导S1回复发光，引起S1的回复发光突变，显著提高了S1的回复发光突变率。其发光突变株具有稳定的遗传学性质。研究表明这种突变是在DNA或DNA的转录水平上发生的。用S1可以简便、快速、灵敏地检测出EB的致突变作用。更多还原

【Abstract】 The luminous tensity of dark variant (S1 ) separated from photobacterium phosphoreum (A2)is less than 1/10000 that of wild-type. Ethidium bromide(EB) (0. 6 ug/ml) can strongly induce dark variant(S1) reversion luminescence, cause reversion mutation and significantly increase reversion rate. These luminous revertants have stable genetical characters. The mutagenesis of EB could be detected with S1 dark variant, and this method was simple, rapid and sensitive.更多还原