【摘要】 为观察细胞培养中细胞凋亡的形态及数量,取4℃环境下不同时间培养的小鼠胸腺细胞,用光镜或吖啶橙(AO),溴化乙锭(EB)染色后荧光镜观察。光镜下可见凋亡小体,荧光镜下早期凋亡细胞发绿色或暗绿色荧光,伴有核结构的变化,而晚期凋亡细胞发橙红色荧光并有凋亡小体的形成。结果表明:光镜下需出现典型核形态变化时凋亡才能确认,AO和EB双重荧光染色后荧光镜检在细胞凋亡检测中既可定性,又可定量。更多还原

【Abstract】 To observe the morphology and amount of apoptosis cells, the authors detected murine thy-mocytes prepared at 4 C with different lengths of time by light microscope or fluoroscope after AO + EB staining. Under light microscope apoptosis bodies were found, while under fluoroscope early apoptosis cells accompanied with nuclear morphologic changes emitting green or dark green fluorescence and late apoptosis cells scintillating reddish orange fluorescence often had apoptosis bodies. The results showed that apoptosis was identified only when the typical change of nuclear morphology appeared under light microscope and that both qualitative and quantitative analysis for determining apoptosis could be done by the double fluorescence staining technique.更多还原