【摘要】 目的：研究脉冲磁场（ＰＥＭＦ）对人早幼粒白血病（ＨＬ－６０）细胞程序性死亡（ＰＣＤ）及周期的影响。方法：以ＨＬ－６０细胞为研究对象，用流式细胞仪进行分析。结果：以ＰＥＭＦ频率为２５０Ｈｚ，场强为５ＭＴ照射ＨＬ－６０细胞，流式细胞仪分析的结果表明，ＰＣＤ呈时间依赖性增加。作用后８ｈ，ＰＣＤ增加到３８．８６％，明显高于对照组。ＰＥＭＦ对ＨＬ－６０细胞周期有影响，随着磁场作用后时间的延长，Ｇ０／Ｇ１期细胞逐渐减少，Ｇ２／Ｍ期细胞逐渐增加。磁场作用后８ｈ，Ｇ０／Ｇ１期细胞减少到４７．４４％，磁场作用后１６ｈ，Ｇ２／Ｍ期细胞增加到４６．５５％。结论：ＰＥＭＦ诱发ＨＬ－６０细胞ＰＣＤ可能与ＰＥＭＦ改变ＨＬ－６０细胞周期有关。更多还原

【Abstract】 Objective:By using flow cytomytry analysis, We studied the effect of pulsing electromagnetic field (PEMF) on apoptosis and cycle in HL60 Methods:The flow cytometric analysis was undertaken. Results: The strength and frequency of PEMF was 5MT and 250Hz respectively It was found that PEMF could increase PCD which showed a time dependent increase 8h after irradiation, PCD in HL60 cells increase to 3886%,being significantly higher than that in the control PEMF could also change cycle in HL60 cellsAs time prolonged,cells of G0/G1 phase reduced and cells of G2/M phase increased significantly 8h after irradiation, cells of G0/G1 phase reduced to 4744%,16h after irradiationed,cells of G2/M phase increased to 4655%Conlusion: The results suggest that mechanism of apoptosis may be related to that PEMF can change cycle in HL60 cells更多还原