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包涵体重组蛋白的纯化及复性

Purification and Refolding of Recombinant Protein as Inclusion Bodies

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【作者】 纪剑飞张成刚

【Author】 Ji Jianfei,Zhang Chenggang

【机构】 中国科学院沈阳应用生态所

【摘要】 综述了近年来国内、外在包涵体重组蛋白纯化及复性研究方面的主要进展.包括包涵体的处理及变性蛋白复性的各种最新技术、手段和方法

【Abstract】 With the development of genetic engineering it has become a routine procedure to produce recombinant mammalian protein from microbial cells.Now more and more genetic recombinant proteins have been cloned to the E.coli and other bacteria in Lab.But most highly expressed recombinant proteins from bacteria always form inclusion bodies,which have no native structures and no biological activities.This is the chief question that the genetic engineering is facing when it comes to the industrial production.In this paper we describe the new methods and development in the research of purification and refolding of recombinant proteins.

【关键词】 包涵体重组蛋白复性纯化
【Key words】 inclusion bodyrefoldingpurification
  • 【文献出处】 沈阳药科大学学报 ,JOURNAL OF SHENYANG PHARMACEUTICAL UNIVERSITY , 编辑部邮箱 ,1998年04期
  • 【分类号】Q503,
  • 【被引频次】116
  • 【下载频次】903
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