【摘要】 该文以PKCR6质粒为载体，构建了人体细胞集落刺激因子（hG－CSF）的次级克隆PKCR6－G－CSF，并成功转染CHO细胞，获得高表达、稳定分泌人G－CSF的转基因细胞，为扩大规模基因工程生产重组细胞因子奠定了一定的基础。更多还原

【Abstract】 The secondary cloning vector PKCR6 carrying human G-CSF cDNA was constructed with PKCR6 plasmid.The CHO cell line Which highly expressed G-CSF gene was successfully obtained, and the stability of cloned CHO supernant is stable. Therefore, this study can be the fundament of producing protein with gene cloning method in large scale for clinical application.更多还原