【摘要】 目的探讨白细胞介素6、8（IL－6、IL－8）以及肿瘤坏死因子（TNF－α）在病毒性脑炎中的作用。方法采用ELISA法检测35例病毒性脑炎和28例健康儿童血清及外周上单个核细胞（PBMC）请生的IL-6、IL-8、TNF－α水平，以及外用血T细胞及亚群。结果病脑患儿血清IL－8、TNF－α水平显著高于对照组，且随病情加重呈增高趋势；体外PBMC诱生IL-6、IL-8、TNF－α水平均明显高于对照组；重型病人TNF－α水平高于轻型病人；病脑组CD3+、CD4+、CD8+细胞百分率及淋巴细胞转化率均较对照组明显降低。结论小儿病脑存在多种细胞因子失调，其中IL－8、TNF－α在其炎症反应及脑损伤过程中可能起重要作用。更多还原

【Abstract】 To explore the effect of interleukin (IL) 6. IL-8 andtumor necrosis factor(TNF)in the pathogenesis of viral encephalitis.Methods Levels of IL-6. IL-8 and TNF-α in serunl and supernatant of peripheral blood mononuclearcytes (PBMC) and T lymphocyte and its subsets were measured in 35 patients with vilal encephalitis and 28 health children.Results Compared with control group, levels of serum IL-8,TNF-α were increased significantly and gradually rised with serioussymptoms in viral encephalitis group. Levels of IL-6, IL-8, TNF-α insupernatant of PBMC were also high in patient group and the level ofTNF-α in serious patients was higher than in common patients. Percentages of CD+3 .CD+4. CD+8 cell and T lymphocyte Proliferative ratewere decreased significantly in patients.Conclusion There exist irregulation of many kinds of cytokin.and which IL-8 and TNF-α may play an important role in process ofinflammatory reaction and brain lesion in viral encephalitis.更多还原