【摘要】 采用逆转录及巢式聚合酶链式反应（ＲＴＰＣＲ）和Ｓｏｕｔｈｅｒｎ杂交方法对肝癌患者的早期血行微转移灶进行检测并做临床随访观察。１０例原发性肝癌患者（ＨＣＣ），经手术切除的癌组织，检测ＡＦＰｍＲＮＡ均为阳性。３９例ＨＣＣ患者中有２４例外周血检测为阳性，阳性率６１．５％。１ａ时间随访到２８例患者，９例阴性结果的病人，１例死亡，死亡率为１１．０％。３例临床证实有转移灶。１９例阳性结果的病人，９例死亡，死亡率为４７．３％，４例临床证实有转移灶。ＲＴＰＣＲ结果不同的两组经卡方检验，Ｐ＜０．０１，患者预后也有明显的差异。检测ＨＣＣ患者外周血中癌细胞是阳性结果，可以说明癌细胞已进入血循环。随访结果显示ＨＣＣ患者外周血中ＡＦＰｍＲＮＡ检测呈阳性则同患者出现血行微转移灶及预后有一定的相关性，可以给临床提供一个肿瘤有远处转移的参考指标。更多还原

【Abstract】 In order to searching an early indicator of hematogenous spreading metastasis of HCC,AFP mRNA was detected by RT PCR and Southen blot in cancer tissues and peripheral blood of patients with HCC,the clinical observation was carried out in 28 cases for 1 year.The results showed that AFP mRNA was found in all of 10 HCC tissues and in 24/39 cases (61.5%) of peripheral blood .During the one year,one patient died (11%) and 3 were found clinically metastasis in 9 AFP mRNA negative cases,nine died (47.3%) and 4 got metastasis in 19 positive cases.In comparison with the two groups,there was singnificant difference in prognosis( P <0.01).It suggests that AFP mRNA in peripheral blood of HCC may be an indicator of circulating HCC cells and associated with HCC hematogenous spreading metastasis.更多还原