【摘要】 突触小泡（ＳｙｎａｐｔｉｃＶｅｓｉｃｌｅ）在神经递质释放过程中起关键性作用。采用膜片钳技术对突触前后细胞同步钳位研究了爪蟾胚胎神经元突触递质释放的过程，提出了如下小泡释放的假说：锚定在突触前膜的小泡中包含两类小泡：锚定态小泡和即发态小泡（即发态小泡定义为钙离子依赖性即时释放的小泡）。后者在动作电位到达时立即释放，而处于锚定态的小泡只有转换为钙离子依赖性释放的即发态时才能进入递质释放程序更多还原

【Abstract】 Synaptic vesicles play a key role during the neural transmitter release. Using current-holding clamp(pre-synapse) and voltage-holding clamp(post-synapse) technology on synapse of the Xenopus laevis embryo neuron cells we studied the transmitter release of the neuron, and developed a hypothesis of the synaptic vesicle release. During Long time (300ms) stimuli on pre-synaptic neuron, we found three different kinds of post-synapse currents, showed that two vesicle docking speeds(τ1=71ms, τ2=20ms) are employed during transmitter release. We established a model to explain the experiments: there are two kinds of vesicles in the docking pool at the pre-synaptic membrane-docking vesicles and Ca2+-depentdent immediate firing vesicles. The latter represent the pool of vesicles immediately available for fusion with the plasma membrane after stimulation. Only after transferring to Ca2+-dependent immediate firing status can docking vesicles be used for release with τ2(20ms). τ1(71ms) describes the speed that the free vesicles dock at the plasma membrane under high-Ca2+ environment.更多还原