【摘要】 以黑曲霉ＪＷ３０３９为出发菌株，通过ＥＭＳ多次诱变处理及最适培养和发酵条件的摸索，使其酸性蛋白酶产量从１８００～２０００ｕ／ｍｌ提高到３３００ｕ／ｍｌ。更多还原

【Abstract】 Two mutants, Aspergillus niger EDF1099 and EDF1120 capable of producing two times as much acid resistant protinase as their parent strain JW3039 of Aspergillus niger were obtained by repeated treatment with EMS. The optimal fermentation medium and fermentation conditions of acid resistant proteinase produced by Aspergillus niger EDF1099 were studied. When Aspergillus niger EDF1099 was cultivated in the optimal fermentation medium and fermentation conditions, its activity was 3375 units/ml (Folin test).更多还原