节点文献
离子交换层析法分离纯化玻璃酸的研究
Study on the Isolation and Purification of Hyaluronic Acid by Ion-exchange Chromatography
【摘要】 选用四种离子交换剂对玻璃酸进行分离纯化研究。结果表明,采用D315大孔离子交换剂,以去离子水为淋洗液、0.6mol/LNaCl溶液为洗脱液,纯化效果较佳。纯化产品杂蛋白含量≤0.3%,粘均分子量>96×104。
【Abstract】 The optimal purification conditions of HA by ion-exchange chromatography were studied.As a result, macroporous ion-exchange rein D3l5 and 0. 6 mol/L NaCl solution as a eluent wereselected. The purification efficiency was very well. The content of protein was less than 0. 3 %. Theaverage molecular weight of HA was more than 960.000.
- 【文献出处】 中国生化药物杂志 ,CHINESE JOURNAL OF BIOCHEMICAL PHARMACEUTICS , 编辑部邮箱 ,1998年05期
- 【分类号】R914
- 【被引频次】11
- 【下载频次】150