【摘要】 构建了α天冬氨酰二肽酶基因（ｐｅｐＥ）与载体ｐＢＶ２２０连接的高表达重组质粒（ｐＢＶＰＥＰ，４３３７ｂｐ）。转化大肠杆菌后，目的基因表达为两种形式的产物，即细胞质中可溶性蛋白（～７５％）和包含体蛋白，共占细菌总蛋白的３０％左右。ＳＤＳＰＡＧＥ分析温敏诱导的表达蛋白，可以观察到约２７ｋＤａ的高表达蛋白条带，与提纯的酶带一致。用不同于以往的萃取包含体蛋白的方法，回收了包含体中９５％以上的表达蛋白。更多还原

【Abstract】 A high expressed recombinant plasmid,consisted of the plasmid pBV220 and the gene of αaspartyl dipeptidase from S.typhimurim TN2401,was constructed and named pBVPEP.After transformed into E.coli DH5 α,the expressed target protein,amounting to 30% of the total cellular proteins,occurred as soluble protein in cytoplasm (about 75%) and also as insoluble inclusion bodies(about 25%).The insoluble protein was recorvered to 95% by mild treatments.The purified protein exhibited a band of 27kDa on SDSPAGE analysis.更多还原