【摘要】 对苏云金芽孢杆菌的培养基配方进行室内摇瓶优化筛选，首先用摇瓶培养筛选到Ⅱ号培养基，在此配方的基础上，将培养基组分划分为氮源、碳源及无机盐三因素，采用三因素二水平正交旋转组合设计的方法进行培养基优化组合研究，建立其芽孢产量依氮源、碳源、无机盐的响应面方程。借助此方程获得响应面最佳点即培养基各组分的最佳配比。实验结果表明，该方法是苏云金芽孢杆菌培养基优化中十分简便、实用、快速的途径。此外，对其间歇发酵过程也进行了初步考察。更多还原

【Abstract】 The No.II media was obtained with shake flask fermentation,and composition of the media was divided into three factors——nitrogen sources,carbon sources and inorganic salts.The relationship between fermentation media and spore numbers of Bacillus thuringiensis was demonstrated by using the Orthogonal rotationg Ocombination test.A response plane equation was formed. The results showed that this method is simple,practical and repid for selecting fermentation media for Bt.In addition,the whole course of batch fermentation was investigated.更多还原