【摘要】 一种新的制备酸性聚丙烯酰胺凝胶的引发系统孙新立＊孙海虹王友爱（河北师范大学生物系，石家庄０５００１６）关键词引发系统；转化率；聚合；酸性凝胶；成胶时间碱性聚丙烯酰胺凝胶电泳已经广泛应用于生化分析的各个领域。但酸性聚丙烯酰胺凝胶电泳、过硫酸铵（ＡＰ）－...更多还原

【Abstract】 A new initiator system for polyacrylamide gel polymerization at low pH is developed. The system consists of ascorbic acid, ferrous sulfate and ammonium persulfate(AP). It is effective under acid conditions even if the concentration of the initiators is very low. To get high incorporation efficiency of acrylamide, the ratio of the AP, ascorbic acid and ferrous sulfate must be in a suitable range. The conversion efficiency has been assessed as a function of the pH of the gelling solution, in pH 2.0-6.58 range. New system is able to guarantee >98% conversion in the pH 2.0-5.0 range. In contrast to the hydrogen peroxideascorbic acidferrous sulfate system widely used under acid conditions, new system gives a much higher incorporation efficiency of acrylamide, lower gel pore size at the same concentration and cross linkage and better gel mechanical property that made the gel easy handle for general purpose. The recommended concentrations of the initiators are: AP, 2.2-3.3 mmol/L, ascorbic acid, 0.91-1.82 mmol/L, FeSO4, 0.046-0.092 mmol/L.更多还原