【摘要】 杜仲成熟干种子经ＧＡ３溶液浸泡后，去掉种胚，切取胚根端胚乳接种在愈伤组织诱导培养基上，５～７ｄ后多数胚乳顶端局部膨大。将膨大部分切下转入成分相同的新鲜培养基上，４０～５０ｄ后形成白色柔软的初生愈伤组织。初生愈伤组织经改造后变为绿色致密具分化能力的愈伤组织，转入分化培养基后出现器官分化，唯正常茎芽分化频率很低，且十分细弱，只有经壮苗培养后才能诱导生根。目前已获得胚乳试管苗２０余株。其无性系中部分植株已在温室内移栽成活。更多还原

【Abstract】 In the present study, dry mature seeds of Eucommia ulmoides were first sterilized and soaked in a sterile GA 3 solution for 24h, and then a small section of radicle end endosperm excised form each seed was inoculated onto callus induction medium. Five through seven days after inoculation,a majority of the inocula swelled up at their tips, each forming a translucent cell mass. At this point it was necessary to cut the swelling portion apart and transfer it onto a fresh medium of the same composition. Hereafter, upon 40￣50 days in culture, ca 40% of the swelling endosperm formed a whitish and soft callus. However, the primary calli were slow growing and showed no potential of redifferentiation. The reformed calli looked greenish, compact and capable of redifferentiation on an appropriate medium. However, most of the regenerants were abnormal, and normal shoot redifferentiation frequency appeared as low as 0.1%￣0.01%. Root induction was conducted by immersing the basal end of the shoot in ABT rooting powder solution after the regenerants were multiplied in vitro and strengthened. So far more than 20 endosperm derived plantlets have been obtained and some of them have survived after being transplanted to soil in the green house. Their performance and chromosome ploidy is to be characterized later.更多还原