【摘要】 为进一步探索Ｐ５３基因与机体发育、肿瘤发生的关系及其与细胞周期中其它调控因子的相互作用，我们采用粘性末端连接法构建了重组野生型Ｐ５３－ＰＸＴ１真核表达载体，并成功地转化Ｃａｃｌ２处理的ＲＲ１细胞，经多种方法鉴定表明其连接率为６９％，转化率为３９×１０３／μｇＤＮＡ。该重组载体的构建无疑将为肿瘤、胎儿畸形及着色性干皮病等的病因及诊治研究提供物质基础更多还原

【Abstract】 To further investigate the relation of P 53 gene to mammals development and tumour occurrence,as well as the effects of P 53 gene in cell cycle,We constructed recombinant wild type P 53 ～PXT 1 expression vector.The screening result of the recommants with several methods suggestted the ligation rate was 69%,its transformation rate was 3.9×10 3/μgDAN.Constructing recombinant P 53 vector will be likely to provide new ideals,new methods and targete moleculcor for diagnosis and therapy of developmental abnormalities,tumour and other diseases.更多还原