【摘要】 切取在ＭＳ培养基中生长５ｄ的河套蜜瓜子叶，在ＭＳ＋ＮＡＡ１＋ＢＡ０．１的培养基中予培养３ｄ后转入芽诱导培养基（ＭＳ＋ＺＴ６）诱导生芽，待芽长２ｃｍ左右转入ＭＳ＋ＩＢＡ０．５诱导生根，１０ｄ后再转入沙质土壤的营养缶本中成苗，整个成苗过程约需６０～７０ｄ，再生植株诱导率可达５５％，为该种植物的遗传转化和优种快速繁殖提供了有利的条件更多还原

【Abstract】 The cotyledon explants of Cucumis melo L.cv Hetau were excised after 5 days of germination on MS medium followed by preculture in MS medium supplemented with NAA 1 mg/L and 6 BA 0.1 mg/L for 3 days.The cotyledon explants were tnnsfered into the shoot inducing medium (MS+Zeatin 6 mg/L) for shoot formation.First shoots were formed after lo days.The generated shoots (2 cm high)were cut and transfered into root inducing medium (MS+IBA 0.5 mg/L)for rooting.The rooted plantlets were transplanted into sandy soil pots for intact plant formation.The total process for in vitro plantlet regeneration takes 60￣70 days.The average regenerated rate is 55%.更多还原