【摘要】 研究了二苯乙烯荧光蓝－Ｓ荧光猝灭测定微量铁（Ⅲ）的实验条件，在ｐＨ５．９的ＨＡｃ－ＮａＡｃ－ＮＨ４Ａｃ缓冲溶液中，试剂的最大激发波长λｅｘ＝３７０ｎｍ，最大荧光发射波长λｅｍ＝４５０ｎｍ；Ｆｅ（Ⅲ）与试剂生成的１∶３的配合物使试剂的荧光强度减弱；利用荧光猝灭法测定微量铁（Ⅲ），线性范围为０～０．４μｇ／１０ｍｌ，方法用于测定茶叶中的微量铁（Ⅲ），结果满意更多还原

【Abstract】  Iron（Ⅲ） in tea was directly determined by using of fluorescence quenching of stilbenefluoblueS（2,2′-Disulfo-4,4′-diaminostilbene-N,N,N′,N′-tetraactic acid disodium salt,C₂2H₂ON₂O14S₂Na₂）.The maximum excitation wavelength of reagent is 370 nm and its maximum emission wavelentth is 450 nm in the HAcNaAcNH4Ac buffer solution at pH5.9.The formation of Fe（Ⅲ）stilbenefluoblueS complex which lead to the decrease of fluorescence indensity of reagent.The liner relationship is obeyed in the range of 0-0.4 μg/10 ml for Fe（Ⅲ）.This method has been applied to the determination of Fe（Ⅲ） in five kinds of tea and the relative standard deviation is 2.2%-2.9%.更多还原