【摘要】 以茉莉酸－牛血清白蛋白复合物作为免疫原，获得了兔抗茉莉酸甲酯抗血清，并在此基础上建立了茉莉酸固相抗原型ＥＬＩＳＡ。此外，利用茉莉酸甲酯和辣根过氧化物酶标记的茉莉酸对鼠抗茉莉酸甲酯单克隆抗体的竞争结合，建立了茉莉酸固相抗体型ＥＬＩＳＡ。上述两种ＥＬＩＳＡ的检测灵敏度分别为０．４２ｐｍｏｌ和０．２０ｐｍｏｌ，检测范围分别为０．９８～１０００ｐｍｏｌ和０．６３～２０ｐｍｏｌ，批内变异系数分别为３．４４％和１．６２％，批间变异系数分别为２．４７％和２．０６％。样品的平均回收率分别为７３．３％和７６．９％。更多还原

【Abstract】 An indirect (antigen immobilized) enzyme linked immunosorbent assay for JA was described. The antiserum was raised in rabbit against (±) JA CO NH Bovine serum albumin (BSA) which was prepared by linking JA to BSA with 1 ethyl 3 ( dimethyl amino propyl) carbodiimide hydrochloride (EDC). JA CO NH Ovalbumin was synthesized via mixed anhydride to coat wells in the indirect ELISA. The ELISA had a detection limit of 0.42 pmol methyl jasmonate, a measuring range 0.98 1 000 pmol, and no verbose purification was required prior to estimation. The average recovery was 73.3%, and the intra and inter assay coefficience of variation of the ELISA was 3.44% and 2.47%, respectively.更多还原