【摘要】 实验研究了ｐＨ值、温度、激活剂（ＥＤＴＡ，Ｃｙｓ）、金属阳离子（Ｋ＋，Ｎａ＋，Ｍｇ２＋，Ｃａ２＋）、去垢剂（ＳＤＳ，ＣＨＡＰＳ，ＴｒｉｔｏｎＸ－１００）以及贮存时间和贮存温度对木瓜蛋白酶活力的影响。结果表明，木瓜蛋白酶具有耐高温、活性强、稳定性好等特征，能耐受一定ｐＨ值和温度变化，对金属离子及去垢剂不敏感。更多还原

【Abstract】 This article studied the effect of several factors on papain activities. When 0 04 mol/L Cys and 0 06 mol/L EDTA were added, the enzyme appeared maximal acitvity. Four positive ions(K +,Na +,Mg 2+ ,Ca 2+ )and three detergents(SDS, CHAPS,Triton X-100 didn’t obviously affect enzyme activity.In the meantime, papain kept more than 50% activity at pH range of 6 8～9 0 and temperature range of 50～80 ℃.Being stored for one month, the enzyme only lost 25% and 60% activity at 4 ℃ and 25 ℃ respectively. These results demonstrate that papain is stable and can be used as additive of beer cleaner and detergent.更多还原