节点文献
磷脂对大鼠离体肠管吸收葛根素的影响
PHOSPHOLIPIDS EFFECT ON ABSORBTION OF PUERARIN IN RATS IN VITRO
【摘要】 目的探讨磷脂(Phospholipids,P)对大鼠离体肠管吸收葛根素(Puerarin,Pur)的影响。方法在改良Fisher-Parsons灌流的大鼠肠管标本中,以HPLC法测定灌流液中Pur的含量。结果Pur的吸收峰在254nm处,Pur的标准曲线呈正相关(r=0.996,P<0.01),标准品的回收率为100.7±4.53%,变异系数为3.25%,在50min时,乳化葛根素和葛根素的扩散常数分别为3×10-7和1×10-7cm2·s-1。结论P能增加Pur的吸收量,提高其生物利用度
【Abstract】 Objective The effect of Phospholipids(P) on the absorbtive content of Puerarin ( Pur) in isolated intestine of rats were investigated. Method At improved Fisher Parsons adevice,the content of Pur and emulsive Pur(EPur) were respectively measured by HPLC.Result The absorbtive peak of wave length of Puerarin was at 250 nm by HPLC.The standard curve of Pur appeared positive interrelating(r=0.996,P<0.01),the standard recovery was 100.7±44.52%, the confficient constant ( cv) was 3. 25%. The content of Pur and ePur were respectively determinated by the method of HPLC in isolated intestine of rats. The diffuse constant D are 1×10 -7 and 3×10 -7 cm 2·s -1 .Conclution The results show that phospholipid can promite absorbtion of Puerarin.
【Key words】 phospholipid; Puerarin; emulsive Puerarin; absorbtion in vitro;
- 【文献出处】 潍坊医学院学报 ,JOURNAL OF WEIFANG MEDICAL COLLEGE , 编辑部邮箱 ,1998年04期
- 【分类号】R285.5
- 【被引频次】17
- 【下载频次】129