【摘要】 目的：探讨肝素对肺成纤维细胞增殖的作用。方法：应用细胞计数、３Ｈ－ＴｄＲ掺入和增殖细胞核抗原（ＰＣＮＡ）细胞免疫组化等方法，检测肝素对培养的Ｗｉｓｔａｒ乳鼠的肺成纤维细胞（ＬＦｂ）的作用。结果：肝素可促进ＬＦｂ增殖且呈量效关系，以０．１ｍｇ·Ｌ－１作用最强，其次为１ｍｇ·Ｌ－１，大于１０ｍｇ·Ｌ－１则表现为抑制效应。肝素可协同ＴＮＦ促ＬＦｂ增殖。结论：低浓度肝素可能直接或与其他因子协同促进ＬＦｂ增殖更多还原

【Abstract】 Purpose To elucidate the effect of heparin on the proliferation of lung fibroblasts （LFb）. Methods Cell count, ^3HTdR incorporation and PCNA immunostaining were used to investigate the effect of heparin on the proliferation of cultured wistar newborn rat LFb. Results Heparin at low concentration （0.1 mg·L^-1, 1 mg·L^-1） stimulated LFb proliferation where as it inhibit the proliferation when concentration was higher than 10 mg·L^-1. The most proliferative effect was at the concentration of 0.1 mg·L^-1. The effect of TNF on stimulating LFb enhanced by heparin. Conclusion Heparin may play a role on lung fibrogenesis by stimulating proliferation of LFb.更多还原