【摘要】 为了进一步研究肺表面活性物质（ＰＳ）的生理功能和今后建立肺表面活性蛋白质Ａ（ＳＰＡ）含量测定，本研究用正丁醇和八－辛－吡喃葡糖酐离心法在羊水中提取和纯化了ＳＰＡ并免疫兔制备了兔抗人抗体。所得ＳＰＡ经免疫电泳及蛋白印迹分析纯度都较佳。鉴于ＳＰＡ在国外已用于抗原测定及在合成磷脂中加入ＳＰＡ可提高对新生儿呼吸窘迫症和成人呼吸窘迫症的疗效，本研究为今后建立ＳＰＡ含量测定，用于临床诊断和治疗奠定了基础更多还原

【Abstract】 In order to make further study on the physiological functions of PS and find a method for measurement of SPA, we have extracted and purified SPA from human amniotic fluid. Pulmonary surfactant was obtained by differential speed centrifugation and SPA was extracted by butanol and then purified by means of ultracentrifugation in OGP extraction. The antibody to SPA were also obtained by immunizing rabbits with SPA. The results of SDS PAGE and western blot showed that we got very pure SPA. Considering that SPA find its application in antigen detection abroad and adding SPA in artificial phospholipid will improve its efficacy, the study will be basis for further SPA detection, clinical diagnosis and therapy.更多还原