【摘要】 目的探讨HGV对家兔感染的敏感性。方法成年兔共进行三代HGV感染实验，除第一代采用人HGV阳性血清外，第二代、第三代分别采用感染的第一、二代兔血清作为感染源，对9只兔（每代3只）进行了传代实验。结果第一、第二、第三代兔血清中都有不同程度生化和肝脏病理改变，除第一代外，其它两代血清的RT－OCR均为阴性，但三代兔肝组织浸出液经消化处理后进行RT－PCR检测全为阳性，对第三代中的一份阳性标本进行NS5区部分核苷酸分析，与美国株（U44403）、西非株GBV－C（U36380）、中国株HGV964和HGVch（U75356）同源性分别为90％、83．5％、92％、97．3％，与江西株比较同源性为96％。结论家兔能被HGV感染，但不是敏感动物。更多还原

【Abstract】 Objcctive To explore the sensitivity of rabbits to HGV infection.Methods Nine rabbits were divided into three groups as three generations used for infec-tious passage experiment of HGV.Rabbits of the first generation were infected with posi-tive serum of patients with HGV.Those of the second generation were infected withserum from the first generation,and those of the third generation with serum taken fromthe second generation.Results Various degrees of biochemcal alteration in serum andpathological changes in liver were found in all experimental rabbits, By RT - PCR, detec-tion of the serum of the first generation showed positive, and the other two generationsnegative,while the digested liver slurry of all rabbits showed positive.The nucleotide se-quence analysis of 5’ non -coding region in one of the mpitive samples of the third gener-ation was assayed and compared with that of the American strain (U44403), West-Africa strain GBV-C(U 36380), Chinese strain HGV 964, HGV ch (U 75356) andJiangxi strain HGV,the homology showed 90%, 83. 5 %, 92%, 97. 5 % and 96 % respec-tively. Conclusions Rabbits can be infected with HGV, but are not sensitive animals.更多还原