【摘要】 在２５００μｍｏｌＥ／ｍ２ｓ光逆境条件下研究了扬麦１５８旗叶类囊体膜、ＰＳⅡ颗粒ＰＳⅡ光化学特性和组分的影响。电子传递活性的结果表明：该麦旗叶类囊体膜及ＰＳⅡ颗粒ＰＳⅡ放氧活性产生下降，其中ＰＳⅡ颗粒的活性下降较为明显。室温荧光发射光谱的结果表明，光逆境导致ＰＳⅡ荧光发射强度的减弱。ＰＳⅡ颗粒多肽组分中，ＯＥＣ３３ｋＤ对光逆境反应较为敏感，降解最为显著。捕光色素蛋白（ＣＰ４３ｋＤ，２８～２９ｋＤ等）也有不同浓度的降解。更多还原

【Abstract】 The effect of light temperature stress on function and structure characteristics of PSⅡ complexs (thylakoid membranes and PSⅡ particles of wheat Yangmai 158 ( T. aestibum L.) flag leaves was studied by measuring the changes of electron transport activities, fluorescence emission spectra and polypeptide components. In flag leaves, light stress (2500 μmol E/m 2s) induced decline of PSⅡ electron transport activities of thylakoid membranes and PSⅡ particles. Light temperature stress treatments decreased the fluorescence emission intensities of PSⅡ particles. When PSⅡ particles of wheat were illuminated with higher light intensity (2500 μmol E/m 2s) at 25 ℃, the extrinsis protein (OEC 33, 23 kD) and the chlorophy11 binding protein CP43 of PSⅡ complex were degraded. It seems that OEC 33 kD polypeotide plays a role of the regulateion on stabilization of the PSⅡ reaction center structure under light stress.更多还原