【摘要】 一氧化氮（ｎｉｔｒｉｃｏｘｉｄｅ，ＮＯ）为活化巨噬细胞（ｍａｃｒｏｐｈａｇｅ，ＭΦ）抑制细胞内弓形虫速殖子增殖的主要效应分子。本文以亚硝基铁氰化钠［ｓｏｄｉｎｍｎｉｔｒｏｐｒｕｓｓｉｄｅ，ＳＮＰ］作为外源性ＮＯ来源探讨其对弓形虫速殖子感染巨噬细胞的作用。结果表明：（１）体外ＳＮＰ的处理，明显抑制了弓形虫速殖子的活力，且经ＳＮＰ预处理的速殖子对ＭΦ的感染力及其侵入细胞后的增殖能力均明显降低，ＳＮＰ的这种抑制作用呈剂量和时间依赖性，并可被肌红蛋白（ＮＯ的清除剂）所逆转；（２）ＳＮＰ可明显抑制ＭΦ内弓形虫速殖子的增殖，并亦呈剂量和时间依赖性，硫酸亚铁、肌红蛋白、Ｌ－半胱氨酸和硫酸亚铁可逆转之。这些结果证明，ＳＮＰ对细胞内和细胞外的弓形虫速殖子的增殖力和活力均具有抑制或杀伤作用。更多还原

【Abstract】 The generation of nitric oxide (NO) is largely responsible for the intracelluar microbiostatic function against Toxoplasma gondii tachyzoite by activated macrophages.The present study was carried out to explore the effect of sodium nitroprusside(SNP) used as exogenous NO on T.gondii tachyzoites infecting macrophages.SNP inhibited both activity and infectivity of the tachyzoites and the multiplication after its invading macrophages,in a dose and time dependent manner.Myoglobin could reverse the inhibitory effect of SNP.In addition,SNP also inhibited the multiplication on the tachyzoites in infected macrophages,in a dose and time dependent manner and the inhibition could be reversed by the addition of myoglobin,FeSO4,or L cysteine and FeSO4.These results indicate that SNP used as a source of exogenous NO contributes to toxoplasmastatic or toxo plasmacidal activity of both intracelluar and extracelluar toxoplasmatic tachyzoites.更多还原