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鼠尾痛刺激、针刺和针刺镇痛后弓状核——导水管周围灰质——延髓头端腹内侧区—氧化氮合酶的变化

CHANGES OF NOS-POSITIVE NEURONS OF ar-PAG-RVM IN RATS FOLLOWING TAIL NOCICEPTIVE STIMULATION, QIAN-LIAO EA AND EAA

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【作者】 李锋范斌周敬修

【Author】 Li Feng, Fan Bin, Zhou JingxiuDepartment of Anatomy, Shanghai Second Medical University 200025 Laboratory of Medical Neurobiology , Shanghai Medical University

【机构】 上海第二医科大学解剖学教研室上医大医学神经生物学国家重点实验室上医大医学神经生物学国家重点实验室 上海200025

【摘要】 SD大鼠分对照、尾部痛刺激、电针和针刺镇痛四组.分别在还原型尼克酰胺嘌吟二核苷酸脱氢酶(NADPH-d)组化下,观察弓状核-导水管周围灰质-延髓头端腹内侧区一氧化氮合酶阳性神经元的变化.结果:上述四组均能见到其阳性神经元和终末,但痛组更显著.痛刺激后15~60分钟,弓状核导水管周围灰质-延髓头端腹内侧区的神经元和终末均有不同程度染色增深.另外,在弓状核处FOS免疫组化和前阿黑皮原原位杂交中,分别在痛刺激后1小时FOS表达才明显增多和痛刺激激后6小时前阿黑皮原基因表达增多.推测一氧化氮可能参与痛觉的传导和诱导c-fos基因,后出现内阿什肽基因表达.

【Abstract】 SD rats were classed into four groups: Control, tail nociceptive stimulation, Qian-Liao EA and EAA. Using the histochemical NADPH-diaphorase staining methods, the activities of NOS in the ar-PAG-RVM of rats were detected. Results were as follows: The NOS-positive neurons and terminals were found in all the four groups, but the activities of NOS-positive neurons and terminals in ar-PAG-RVM increased to a different extent 15’ - 60’ after the stimulation. However, FOS-like immunoreactive neurons in the ar were obviously detected 60’ after the stimu-lation by means of immunocytochemistry, and POMC mRNA expression in the ar were obviously found 6 hrs after the stimulation by means of situ hybridization histochemistry. The results sug-gested that no might be involved in pain processing and the induction of expression of c-fos, and that c-fos possibly modulated expression of POMCmRNA.

【基金】 国家自然科学基金资助项目(39230360)
  • 【文献出处】 解剖学杂志 ,Chinese Journal of Anatomy , 编辑部邮箱 ,1998年05期
  • 【分类号】R245.3
  • 【被引频次】6
  • 【下载频次】92
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