【摘要】 利用细胞培养技术，Ｃａ２＋敏感荧光探针Ｆｌｕｏ－３／ＡＭ标记细胞，激光扫描共聚焦显微镜检测鸡胚胎巨噬细胞内游离钙浓度（〔Ｃａ２＋〕ｉ）的变化。结果显示细菌脂多糖（ＬＰＳ）可引起培养的鸡胚胎巨噬细胞内〔Ｃａ２＋〕ｉ升高，ＬＰＳ的作用可被维拉帕米部分抑制。本实验表明激光扫描共聚焦显微镜结合Ｆｌｕｏ－３／ＡＭ能准确迅速地检测单个或多个贴壁粘附细胞胞内〔Ｃａ２＋〕ｉ的动态变化，并同步观察细胞的形态及其荧光分布情况。更多还原

【Abstract】 Using laser scanning confocal microscopy and Fluo-3/AM as a fluorescence probe,intracellular free calcium concentrations(〔Ca2+〕i) in culture macrophages of chick embryos during treated with LPS,Verapamil+LPS were measured.It was found that LPS causes an increase of intracellular 〔Ca2+〕i,which were inhibited partly by calcium channel blocker verapamil in cultured macrophages of chick embryos.laser scanning Confocal microscopy and Fluo-3/AM could measure the changes of intracellular 〔Ca2+〕i and observed cell morphology and fluorescence distribution at the same time.更多还原