【摘要】 为了检测贝类标本中的甲型肝炎病毒，我们建立了敏感特异的免疫捕获—逆转录—聚合酶链反应技术（ＩＣ－ＲＴ－ＰＣＲ）。应用该技术检测不同地区采集的贝类标本中的ＨＡＶ－ＲＮＡ后发现：４６份贝类标本有２９份为阳性，检出率达６３０４％（２９／４６），其中毛蚶为６３１６％（１２／１９），文蛤为９０００％（９／１０），蚶子为５４５５％（６／１１），说明各种贝类有不同程度的污染；南部、中部地区贝类标本中的ＨＡＶ－ＲＮＡ检出率较高，北部地区１０份标本仅检出１份阳性，这与当地甲肝发病率较低一致。更多还原

【Abstract】 specimens of shellfish collected from 3 points of Jiangsu coast were detected for HAV RNA by using Immune Capture-Reverse Transcriptase-polymerase Chain Reaction (IC-RT-PCR)in 1993. 29 specimens of shellfish were positive for HAV RNA, the general positive rate was 63.04%(29/46). Of which, 63.16%(12/19) was found in Arca subcrenate, 90.0%(9/10)in Meretrix and 54.55%(6/11) in blood clam. So, it showed that many kinds of shellfish were contaminated by HAV in these areas of Jiangsu Province. The data also indicated that the positive rates of HAV RNA were 78.9% and 76.47% in both southern point A and middle point B, which were more higher than the north point C (only 10%). This pattern of contribution of HAV RNA was accorded with the contribution of HAV patients in Jiangsu Province.更多还原