【摘要】 采用原位杂交和免疫组化技术，对１０只７周龄雄性ＳＤ大鼠双侧髁突中胰岛素样生长因子Ⅰ（ＩＧＦ－Ⅰ）及其ｍＲＮＡ表达进行研究。结果发现：生长期大鼠髁突软骨中ＩＧＦ－Ⅰ及其基因均有表达，但ＩＧＦ－Ⅰ阳性强度在生发层中最强，而ＩＧＦ－ⅠｍＲＮＡ在过渡层和成熟层中最强。表明髁突软骨具有局部合成和分泌ＩＧＦ－Ⅰ的能力，可介导生长激素的命令式作用，其差异性表达建立起局部反应的反馈环路，为生长伺服理论提供了直接证据更多还原

【Abstract】 It was studied the expression of IGFⅠ and its mRNA in the condylar cartilage of 10 7weekold SD male rats by using in situ hybridization and immunohistochemistry technique. The results showed both IGFⅠ and its gene expressed in growing rat condyle. IGFⅠ peptide was abundant in germinal zone, and positive reaction of its mRNA was strongest in transitional and maturational zones. These indicate that condylar cartilage has the capability of local production and secretion of IGFⅠ, mediating the command efffect of STH, and differential expression of IGFⅠ and its mRNA might establish the local feedback loop, which supply a direct evidence for servosystem theory of facial growth.更多还原