【摘要】 为了解妇科肿瘤患者CD3AK细胞的表型特征，并探讨来源于妇科良、恶性肿瘤患者CD3AK细胞表型特征的异同，采用间接免疫荧光法测定良、恶性妇科肿瘤患者CD3AK细胞的CD3，CD4，CD8阳性细胞率。结果表明：①妇科恶性肿瘤患者CD3AK细胞于培养第1，2周，CD3，CD4和CD8阳性细胞率均较高（40．25％～61．36％），培养第3周，CD3，CD4和CD8阳性细胞率均有不同程度下降，尤以CD₈^＋细胞下降明显（第1周为52．96％，第2周为40．24％，第3周为25．72％）；②妇科良性肿瘤患者CD3AK细胞CD3，CD4和CD8阳性细胞率在培养第1周均较高（39．86％～59．84％），培养第2，3周均有降低，以CD₄^＋细胞下降更明显（第1周为50．52％，第2周为41．91％，第3周为29．97％）；③妇科良、恶性肿瘤CD3AK细胞CD3，CD4，CD8细胞阳性率除培养第1周恶性肿瘤组CD8细胞阳性率显著高于良性肿瘤组外（P＜0．05），余均无显著性差异（P＞0．05）。结果提示：CD3AK细胞是一异质细胞群，不同来源或相同来源不同功能状态的CD3AK细胞在体外受到刺激后，不同亚群细胞可呈选择性增殖，从而表现出不同的表型特征。更多还原

【Abstract】 In other to study the phenotype characteristics of CD3AK cells and the difference of CD3AK cell phenotypes between benign and malignant gynecological tumors, the positive rates of CD3, CD4 and CD8 of CD3AK cells were determined using the indirect immunofluoreacence technique. The results showed that:①in malignant gynecological tumor group, the positive rates of CD3, CD4 and CD8 of CD3AK cells were high (40. 25% ~ 61. 36% ) at the 1st and 2nd week of culture, and then decreased in different degrees at the 3rd week; especially, the CD8 positive rate reduced obviously (1st week 52. 96%, 2nd week 40. 14%, 3rd week 25 .72% ); ②in benign gynecological tumor group, the positive rates of CD3, CD4and CD8 of CD3AK cells increased at the 1st week (39. 86% ~ 59. 84% ), but all of them decreased at the 2nd and 3rd week, especially, the CD4 positive rate reduced obviously (the 1st week 50. 25%, the 2nd week 41 .91 %, the 3rd week 29.97% ); ③the positive rates of CD3, CD4, CD8 of CD3AK cells in malignant gynecological tumors group were significantly higher (P < 0. 01 ) than those in the benign gynecological tumor group at the 1st week of culture. However, there were no significant difference between benign and malignant gynecological tumor groups in other culture time (P > 0. 05). Above results support the viewpoint that CD3AK cell is a different properity cell group. It shows that CD3AK cells, which generate from different patients, are selective proliferation as stimulated in vitro, and present different phenotype characteristics.更多还原