节点文献
ELISA和PCR法在研究乙肝病毒传播途径中的应用
Application of ELISA and PCR in study of the mode of transmission of hepatitis B virus
【摘要】 应用HBV表面抗原酶标试剂盒和诊断HBVDNA的PCR试剂盒分别检测可能污染有HBV的不同来源标本30份,结果ELISA法检出HBsAg阳性5例,检出率16.7%;PCR法检出HBVDNA阳性12例,检出率40%,明显高于ELISA法,提示应用PCR技术探讨乙肝病毒传播途径似乎效果更好。
【Abstract】 Polymerase chain reaction(PCR) technique was used to study hepatitis B virus (HBV) DNA, and enzyme-linked immune sorbent assay (ELISA) was adopted to detect HBsAg in the 30 different samples in which 12 cases were HBVDNA positive by ELISA,with rate of 40% and 5 were HBsAg positly by ELISA,with rate of 16. 7%. This result indicates that the PCR superior to the ELISA in detection of hapertitis B virus.
【关键词】 酶联免疫吸附试验;
聚合酶链反应;
乙型肝炎病毒;
【Key words】 enzyme-linked immune sorbent assay; polymerase chain reaction; hepatitis;
【Key words】 enzyme-linked immune sorbent assay; polymerase chain reaction; hepatitis;
- 【文献出处】 淮南矿业学院学报 , 编辑部邮箱 ,1998年02期
- 【分类号】R373
- 【下载频次】38