节点文献

ELISA和PCR法在研究乙肝病毒传播途径中的应用

Application of ELISA and PCR in study of the mode of transmission of hepatitis B virus

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 张荣波; 王克霞; 王健; 张宁;

【Author】 Zhang Rongbo; Wang Kexia; Wang Jian; Zhang Ning (Medical College) (Huainan Railway Hospital) (Medical College)

【机构】 淮南工业学院医学分院; 淮南铁路医院;

【摘要】 应用HBV表面抗原酶标试剂盒和诊断HBVDNA的PCR试剂盒分别检测可能污染有HBV的不同来源标本30份,结果ELISA法检出HBsAg阳性5例,检出率16.7%;PCR法检出HBVDNA阳性12例,检出率40%,明显高于ELISA法,提示应用PCR技术探讨乙肝病毒传播途径似乎效果更好。

【Abstract】 Polymerase chain reaction(PCR) technique was used to study hepatitis B virus (HBV) DNA, and enzyme-linked immune sorbent assay (ELISA) was adopted to detect HBsAg in the 30 different samples in which 12 cases were HBVDNA positive by ELISA,with rate of 40% and 5 were HBsAg positly by ELISA,with rate of 16. 7%. This result indicates that the PCR superior to the ELISA in detection of hapertitis B virus.

  • 【分类号】R373
  • 【下载频次】38
节点文献中: 

本文链接的文献网络图示:

本文的引文网络