【摘要】 为全面评价烹调烟雾的危害，收集居民家用脱排油烟机中的烹调烟雾冷凝物，经干燥脱水后，应用人外周血淋巴细胞非程序ＤＮＡ合成试验（ＵＤＳ）及ＢＡＬＢ／ｃ３Ｔ３细胞体外培养系统，检测居民厨房中烹调烟雾冷凝物的ＤＮＡ损伤作用及对ＢＡＬＢ／ｃ３Ｔ３细胞的毒性与转化作用。结果表明受试物在加Ｓ９和不加Ｓ９时，均能提高ＣＰＭ值，ＤＮＡ放射比活性Ｒ值均随测试浓度的增加而升高；受试物对ＢＡＬＢ细胞具有明显的细胞毒性与转化作用，其转化率随检测浓度升高而升高。提示烹调烟雾冷凝物具有ＤＮＡ损伤和细胞转化作用，且具有一定的剂量效应关系。更多还原

【Abstract】 To assess the hazardous effect of cooking fume comprehensively,cooking fume condensates were collected from many families that used rapseed oil as cooking oilsUnscheduled DNA synthesis(UDS) test and the cell transformation assay were applied to detect the DNA damage and the cell transforming activity of cooking fume condensatesThe results showed that 1)the condensates of cooking fumes could increase the CPM of human peripheral blood lymphocytes apparently with and without S9,and there was a significant doseeffect relationship between the CPM and consistency of condensates 2)condensates of cooking fumes ould induce morphological transformation in BALB/c3T3 A3111 cells,and there was a doseeffect relationship in the cell transformation frequencyIt indicated that condensates of cooking fumes ould cause DNA damage and cell transforming activity,and doseeffect relationship in certain extent was induced cooking fumes might possess potential carcinogenic hazards to the exposures更多还原