节点文献

抗-CEA单抗的188Re标记

Preparation of Re labelled antibodies

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 朱明花曹蓉珍李文新盛荣何蔚瑜周伟汪勇先

【Author】 ZHU Minghua CAO Rongzhen LI Wenxin SHENG RongYIN Duanzhi HE Weiyu ZHOU Wei WANG Yongxian(Shanghai Institute of Nuclear Research,The Chinese Academy of Sciences, Shanghai 201800)

【机构】 中国科学院上海原子核研究所

【摘要】 采用转移能力更强的葡萄糖酸钠和SnCl2的混合液及Na188ReO4,用直接标记法标记了抗-CEA单抗。研究了影响标记率的若干因素,如抗体和抗坏血酸的用量、亚锡的浓度、反应液pH值以及反应时间等,探索出标记率可达80%以上的标记条件。

【Abstract】 A simple technique of directly labelling antibodies with Re has been developed.The reduction of antibody disulfide groups was achieved by incubation of antibodywith ascorbic acid (pH=6.5)for an hour at room temperature and a solution of excessSnCl2 in sodium gluconate was added to the AA-reduced antibody followed by theaddition of perrhenate,Some factors that influence labelling efficiency,such as thepH of the reaction mixture, the labelling time,and the amount of antibodies andreductive agent,were studied experimentally and a better labelling method wasestablished,The labelling yidlds,as determined by paper chromatography,weregreater that 80%.

【关键词】 188Re抗体标记
【Key words】 ReAntibodyRadiolabelling
【基金】 国家自然科学基金,IAEA部分资助
  • 【分类号】TL923
  • 【被引频次】2
  • 【下载频次】23
节点文献中: 

本文链接的文献网络图示:

本文的引文网络