【摘要】 报道了玉米醇溶蛋白基因启动子的亚克隆以及利用该启动子和ＧＵＳ基因来构建表达质粒ｐＨＺＰ—ＧＵＳ．经过部分降解和亚克隆，得到玉米醇溶蛋白基因启动子，将该启动子与含ＧＵＳ基因编码序列和ＮＯＳ终止子的ＢａｍＨⅠ片段连接，构成融合基因．将该融合基因克隆到含有ＨＰＴ筛选标记基因的质粒ｐＧＬ２中，得到表达质粒ｐＨＺＰ—ＧＵＳ．更多还原

【Abstract】 Subcloning of a zein gene promoter and construction of an expression plasmid containing a fusion gene consisting of this promoter and GUS gene were reported. A 938 bp promoter fragmant derived from a cloned 7 7 kb maize DNA fragment was subcloned into EcoR Ⅰ—Rsa Ⅰ sites of pUC19.Ligation reaction between this promoter and a BamH Ⅰ DNA fragment containing the coding sequence of GUS gene and NOS-terminator generates the fusion gene. Plasmid pGL2 with HPT selecting marker was used as the cloning vector of the fusion gene to create the expression plasmid pHZP-GUS.更多还原